MiraCLEAN® Endotoxin Removal Kit

A convenient and effective method for the removal of endotoxin from plasmid DNA preparations

Product No. Quantity Price Add to

MiraCLEAN®-Endotoxin Removal Kit

MIR 5910

for 10 mg DNA

$139.00

MIR 5900

for 100 mg DNA

$415.00

To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.

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Effectively Removes Endotoxin from Plasmid Samples - Aids in the safety and efficiency of gene delivery research.
Compatible With In Vivo and In Vitro Applications - Removes harmful endotoxin that could cause decreased transfection efficiencies In Vitro and inflammatory reactions In Vivo .
Ease of Use - Simple phase separation protocol allows quick visualization of recovered nucleic acid preparations.

Data

Figure 1. The MiraCLEAN Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per mg of DNA (EU/mg). The DNA was treated with the MiraCLEAN Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.

Figure 2. Plasmid DNA Exhibits High Transfection Efficiency after Treatment with the MiraCLEAN Kit. COS-7 cells were transfected with 2 µg of plasmid DNA and 2µl of the TransIT-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression.