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MiraCLEAN®-Endotoxin Removal Kit

A convenient and effective method for the removal of endotoxin from plasmid DNA preparations

Product No.QuantityPriceAdd to
MIR 5910for 10 mg DNA$127.00
MIR 5900for 100 mg DNA$383.00
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.
 

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  • Effectively Removes Endotoxin from Plasmid Samples—Aids in the safety and efficiency of gene delivery research.
  • Compatible With In Vivo and In Vitro Applications—Removes harmful endotoxin that could cause decreased transfection efficiencies in vitro and inflammatory reactions in vivo.
  • Ease of Use—Simple phase separation protocol allows quick visualization of recovered nucleic acid preparations.

Data

Figure 1 Figure 1. The MiraCLEAN Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per mg of DNA (EU/mg). The DNA was treated with the MiraCLEAN Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.
Figure 2
 
Figure 2. Plasmid DNA Exhibits High Transfection Efficiency after Treatment with the MiraCLEAN Kit. COS-7 cells were transfected with 2 µg of plasmid DNA and 2µl of the TransIT-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression.

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