TransIT®-LT1 Transfection Reagent

Figure 1. The TransIT®-LT1 Reagent Efficiently Delivers DNA to a Wide Variety of Cell Lines. Using the TransIT®-LT1 Transfection Reagent, cells were transfected with the pEGFP-C1 expression vector, and the percentage of EGFP expressing cells was determined 24-48 hours post-transfection.

Figure 2. The TransIT®-LT1 Reagent Exhibits Low Cellular Toxicity Compared to Another Leading Transfection Reagent. COS-7 cells were transfected using Mirus Bio’s TransIT®-LT1 Reagent or a leading competitor, Reagent L, according to the manufacturer’s recommendations. The presence of rounded cells and the disappearance of many cells in the Reagent L transfected sample, compared to the nontransfected cells (A), illustrates the extreme cellular toxicity observed with Reagent L (C). In contrast, the cells transfected with the TransIT®-LT1 Reagent (B) exhibited minimal cellular toxicity.

Figure 3. The TransIT®-LT1 Reagent Performs Best in the Presence of Serum Containing Media. Using the TransIT®-LT1 Reagent, the indicated cells lines were transfected with a luciferase expression vector using three different media conditions: cells were transfected in the presence of (1) serum-free media or (2) complete media with media changes 4 hours post-transfection, or transfected in the presence of (3) complete media with no media change post-transfection. The cells were harvested 48 hours post-transfection, and the total luciferase activity per well was determined.