TransIT®-LT1 Transfection Reagent

A broad spectrum, low toxicity, DNA transfection reagent

Comparable performance to FuGENE® 6 Reagent, but at a lower cost
TransIT®-LT1 outperforms Lipofectamine™ 2000
Superior performance to PolyFect® at a lower cost

• Broad Spectrum DNA Delivery—Utilize one transfection reagent and protocol for a variety of cells.
• Low Cellular Toxicity—Maintain cell density and reduce experimental biases.
• High Efficiency Delivery—Achieve expression in a large population of cells for experimental success.
• Deliver Single or Multiple Plasmids—Suitable for many applications such as gene expression, siRNA/shRNA expression, viral production, and promoter analysis.

Cell Types Successfully Transfected by Mirus Bio:
A549, BNL.CL2, BRL-3A, C2C12, *C6, *CHO-K1, Clone 9, *COS-1, *COS-7, *Daoy, DBTRG-05MG, *DI-TNC1, *DU 145, *HEK 293, *HeLa, Hepa 1-6, Hepa 1cLc7, HepG2, HLF-a, Huh-7, *HUVEC, *Jurkat, *K562, KB, KLN 205, *LL/2 (LLC1), *LNCaP-FGC, MCF-7, *Neuro-2a, *NIH3T3, OVCAR3, *PC3, *PC-12, *primary human astrocytes, *primary human chondrocytes, primary mouse hepatocytes, primary rat hepatocytes, *primary human keratinocytes, *RAW 264.7, *SK-N-MC, SKOV3, *SVGp12, SW900, *THP-1, Vero, and WRL-68.

*Higher transfection efficiencies can be achieved using Mirus Bio’s TransIT® Cell Line Specific Reagents.

Data

Figure 1: The TransIT®-LT1 Reagent Efficiently Delivers DNA to a Variety of Cell Lines.
Figure 2: The TransIT®-LT1 Reagent Exhibits Low Cellular Toxicity Compared to Another Transfection Reagent.
Figure 3: The TransIT®-LT1 Reagent Performs Best in the Presence of Serum Containing Media.

Figure 1. The TransIT®-LT1 Reagent Efficiently Delivers DNA to a Wide Variety of Cell Lines. Using the TransIT®-LT1 Transfection Reagent, cells were transfected with the pEGFP-C1 expression vector, and the percentage of EGFP expressing cells was determined 24-48 hours post-transfection.

A-Nontransfected B-TransIT®-LT1 Reagent C-Competitor Reagent L   Figure 2. The TransIT®-LT1 Reagent Exhibits Low Cellular Toxicity Compared to Another Leading Transfection Reagent. COS-7 cells were transfected using Mirus Bio’s TransIT®-LT1 Reagent or a leading competitor, Reagent L, according to the manufacturer’s recommendations. The presence of rounded cells and the disappearance of many cells in the Reagent L transfected sample, compared to the nontransfected cells (A), illustrates the extreme cellular toxicity observed with Reagent L (C). In contrast, the cells transfected with the TransIT®-LT1 Reagent (B) exhibited minimal cellular toxicity.

Figure 3. The TransIT®-LT1 Reagent Performs Best in the Presence of Serum Containing Media. Using the TransIT®-LT1 Reagent, the indicated cells lines were transfected with a luciferase expression vector using three different media conditions: cells were transfected in the presence of (1) serum-free media or (2) complete media with media changes 4 hours post-transfection, or transfected in the presence of (3) complete media with no media change post-transfection. The cells were harvested 48 hours post-transfection, and the total luciferase activity per well was determined.