TransIT®-EE Delivery Solution and Starter Kit

An "Enhanced Expression" in vivo delivery solution that delivers DNA to the livers of laboratory mice using hydrodynamic tail vein injection

• Naked Nucleic Acid Delivery—Achieve increased levels of gene expression than compared to TransIT-QR Hydrodynamic Delivery Solution, TransIT-In Vivo Gene Delivery System, polylysine and PEE methods.
• Versatile Platform—Suitable for DNA delivery using the hydrodynamic tail vein injection to the mouse strain of your choice.

Starter Kit Components:

Each starter kit contains the TransIT-EE Hydrodynamic Delivery Solution, 10 syringes, needles and alcohol swabs; and a mouse restraint device designed specifically for tail vein injections. The TransIT-EE Hydrodynamic Delivery Solution is also sold separately as indicated above.

Data

Figure 1. TransIT-EE Solution Leads to Enhanced Luciferase Expression in the Liver. TransIT-QR or TransIT-EE Hydrodynamic Delivery Solutions were used to deliver 10 µg of a luciferase expression vector to mice using the hydrodynamic delivery tail vein injection procedure. Twenty-four hours post-injection the livers were harvested and assayed for luciferase activity.

Figure 2. TransIT-EE Solution Leads to Enhanced Lucifrease Expression in a Variety of Organs. TransIT-QR or TransIT-EE Hydrodynamic Delivery Solutions were used to deliver 10 µg of pCI-Luc (CMV promoter-driven luciferase expression vector) to mice using the hydrodynamic tail vein injection procedure. Twenty-four hours post-injection the organs were harvested and assayed for luciferase activity. NOTE: Following gene delivery via a tail vein injection, the highest level of transgene expression is found in the liver, with reduced levels of expression found in the spleen, lung, heart and kidneys.

NOTE: In vivo hydrodynamic delivery of non-viral nucleic acids is covered by worldwide patents and patent applications of Mirus Bio Corporation, including U.S. Patent 6,627,616, U.S. Patent 6,379,966, and related filings worldwide. Purchase of this product does not provide a license to this delivery technology, which is required for all research and commercial uses by for-profit entities. To inquire about a license, please contact Mirus Bio Corporation.