TransIT®-EE Delivery Solution and Starter Kit

An "Enhanced Expression" In Vivo delivery solution that delivers DNA to the livers of laboratory mice using methods including hydrodynamic tail vein injection

Product No. Quantity Price Add to

TransIT®-EE Delivery Solution (all three vectors)

MIR 5340

40 injections

$195.00

TransIT®-EE Starter Kit

MIR 5310

10 injections

$111.00

To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.

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Naked Nucleic Acid Delivery - Achieve increased levels of gene expression than compared to TransIT-QR Delivery Solution, TransIT- In Vivo Gene Delivery System, polylysine and PEI methods.
Versatile Platform - Suitable for DNA delivery using methods including hydrodynamic tail vein injection to the mouse strain of your choice.

Starter Kit Components:
Each starter kit contains the TransIT-EE Delivery Solution, 10 syringes, needles and alcohol swabs; and a mouse restraint device designed specifically for tail vein injections. The TransIT-EE Delivery Solution is also sold separately as indicated above.

Data

Figure 1. TransIT-EE Solution Leads to Enhanced Luciferase Expression in the Liver. TransIT-QR or TransIT-EE Delivery Solutions were used to deliver 10 µg of a luciferase expression vector to mice using the hydrodynamic delivery tail vein injection procedure. Twenty-four hours post-injection the livers were harvested and assayed for luciferase activity.

Figure 2. TransIT-EE Solution Leads to Enhanced Lucifrease Expression in a Variety of Organs. TransIT-QR or TransIT-EE Delivery Solutions were used to deliver 10 µg of pCI-Luc (CMV promoter-driven luciferase expression vector) to mice using the hydrodynamic tail vein injection procedure. Twenty-four hours post-injection the organs were harvested and assayed for luciferase activity.

NOTE: Following gene delivery via a tail vein injection, the highest level of transgene expression is found in the liver, with reduced levels of expression found in the spleen, lung, heart and kidneys.