TransIT® In Vivo Gene Delivery System

Figure 1. Beta-Galactosidase Expression in Mouse Liver is Achieved Using the TransIT In Vivo Gene Delivery System. A B-Gal expression vector was complexed with the TransIT In Vivo Polymer Solution and delivered to mice using the hydrodynamic tail vein injection. Twenty-four hours post-injection, liver sections were stained using the Beta-Gal Staining Kit.

NOTE: Following gene delivery via a tail vein injection, the highest level of transgene expression is found in the liver, with reduced levels of expression found in the spleen, lung, heart and kidneys.

Figure 2. The TransIT In Vivo Gene Delivery System Leads to Luciferase Expression in a Variety of Organs. A luciferase expression vector was complexed with TransIT In Vivo Polymer and delivered to mice using the hydrodynamic tail vein injection. Luciferase expression was measured twenty-four hours after injection.

Figure 3. Quantitative Comparison of Luciferase Reporter Activity in Mouse Liver Following In Vivo Gene Delivery. A luciferase expression vector was complexed with either the TransIT In Vivo Polymer Solution or the indicated agent and delivered to mice via tail-vein injection. Luciferase expression was quantified by assaying the total amount of luciferase produced in mouse liver.