NEW! TransIT^®-2020 Transfection Reagent

• Superior Performance - TransIT^®-2020 outperforms FuGENE^® HD, Lipofectamine™ 2000, and Lipofectamine™ 2000 CD.
• Broad Spectrum DNA Delivery - Achieve high expression in many cell types, including hard to transfect cells.
• Animal Origin Free - TransIT^®-2020 provides high performance with maximum compatibility.
• Scientifically Proven - Developed by Mirus Bio scientists with over 15 years of gene delivery expertise.

Cell Types Successfully Transfected at Mirus

C6, COS7, CHO-K1, Daoy, DB-TRG-05MG, DI-TNC1, HEK 293, HeLa, HepG2, Jurkat, K562, Neuro-2a, NIH-3T3, Vero, primary HSAEpic, and primary MEFs

Cell Types Successfully Transfected by Other Researchers

Cell lines: 2b4.11, A549, C3H/10T1/2, CCD18Co, CHO.K1, Colon38, HCT116, HEK-293, HEK-293T, HPTCs, INS-1, J774A.1, KYSE-410, LS-180, MCF-7, MDA-MB-231, Monc-1, NCCIT, NE1, NIH3T3, NRK52E, NSCLC cell line H522, PC-12, Phoenix Eco, RAW 264.7, RGC-5, Saos-2, SH-5YSY, SV40-Large T antigen rat aortic smooth muscle cells, T-98G, TE8 and U2OS
 
Primary and stem cells: human ES cell derived neural progenitors, human mesenchymal stem cells, mouse ES cell derived cardiomyocytes, primary human airway epithelial cells, primary human astrocytes, and primary MEFs

Data

High Performance Plasmid Transfection. Primary Human Small Epithelial Cells (HSAEpic) were transfected using TransIT-2020 and an EGFP expression plasmid (4:1 reagent-to-DNA ratio). Images were taken 24 hours post-transfection using a Zeiss axiovert inverted fluorescence microscope.

Superior Gene Expression in a Broad Spectrum of Cell Types.
Experimental Details: Luciferase gene expression was compared in HEK 293, K562, CHO-K1, and primary MEF cells transfected with a luciferase expression plasmid using TransIT-2020 (Mirus Bio, 3:1 reagent to DNA ratio), FuGENE HD (Roche, 7:2 reagent to DNA ratio), Lipofectamine™ 2000 (Invitrogen, 5:2 reagent to DNA ratio), and Lipofectamine™ 2000 CD (Invitrogen, 5:2 reagent to DNA ratio). Transfections were performed in 24-well plates using 0.5 µg of plasmid DNA per well. The optimal level of each transfection reagent was determined empirically and all reagents were used according to manufacturer’s protocol. Cells were harvested at 24 hours post-transfection and assayed for luciferase activity. Error bars represent the standard deviation of triplicate wells.