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TransIT-siQUEST® Transfection Reagent

A high efficiency, low toxicity, siRNA transfection reagent for mammalian cells

Product No.QuantityPriceAdd to Cart
TransIT-siQUEST® Transfection Reagent
MIR 21140.4 ml$250.00
MIR 21101 ml$402.00
MIR 21155 x 1 ml$1,755.00
MIR 211610 x 1 ml$3,245.00
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.

Resources

Protocol
MSDS
Technical Report: High Efficiency siRNA Delivery In Vitro
Publication: Delivery of siRNAs into Mammalian Cells...
Poster: RNAi Applications in Nucleic Acid Delivery...
Frequently Asked Questions
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Tips From the Bench
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  • Broad Spectrum siRNA Delivery - Utilize one transfection reagent and protocol for a wide variety of cells.
  • Low Cellular Toxicity - Maintain cell density and reduce experimental biases.
  • Reproducible Results - Obtain consistent, targeted gene knockdowns from day to day.
  • High Knockdown Efficiency - Achieve optimal gene silencing in a large percentage of cells to ensure experimental success.
Cell Types successfully transfected at Mirus using TransIT-siQUEST Transfection Reagent
 

Customer Testimonial

Dr. Jagadananda Ghosh
Henry Ford Health System - Vattikuti Urology Institute
“Our lab routinely uses Mirus Bio transfection products, and we recently published using TransIT-siQUEST to perform RNA interference experiments in LNCaP cells to elucidate the role of p53 in selenium-induced apoptosis in prostate cancer cells. These results were published in 2010 in the International Journal of Oncology." (Sarveswaran, S. et al. IJO. Jan 2010)

Data

Figure 1: High Knockdown and Low Toxicity Using TransIT-siQUEST® Reagent in CHO Cells Stably Expressing Firefly Luciferase.
Figure 2: Inhibition of PPAR-alpha Expression in Primary Mouse Hepatocytes Using the TransIT-siQUEST Reagent.
Figure 3: Inhibition of Stably Expressed Firefly Luciferase Using the TransIT-siQUEST Reagent.
Figure 4: Efficient Target Gene Knockdown in Selected Cell Lines Using TransIT-siQUEST Reagent.
Figure 5: Visualization of Fluorescently-Labeled siRNA Transfected with the TransIT-siQUEST Reagent.

Figure 1:High Knockdown and Low Toxicity Using TransIT-siQUEST® Reagent in CHO Cells Stably Expressing Firefly Luciferase.
Figure 1. High Knockdown and Low Toxicity Using TransIT-siQUEST® Reagent in CHO Cells Stably Expressing Firefly Luciferase. CHO-luc cells were grown in 24 wells plates and transfected with 25 nM of either a non-targeting siRNA or a anti-firefly luciferase siRNA using the indicated reagents with the volumes noted beneath each well. Luciferase expression, normalized to non-targeting siRNA control (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as percent cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph.
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Figure 2:Inhibition of PPAR-alpha Expression in Primary Mouse Hepatocytes Using the TransIT-siQUEST Reagent.
Figure 2. Inhibition of PPAR-alpha Expression in Primary Mouse Hepatocytes Using the TransIT-siQUEST® Reagent. Primary mouse hepatocytes were transfected with an anti-PPAR-alpha siRNA or a non-targeting control siRNA using the TransIT-siQUEST® Reagent. Twenty-four hours post-transfection, the amount of PPAR-alpha mRNA was measured relative to GAPDH mRNA levels using qRT-PCR and then scaled to the expression level of the specific target mRNA in the Opti-MEM® media only (untreated) control.
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Figure 3:Inhibition of Stably Expressed Firefly Luciferase Using the TransIT-siQUEST Reagent.
Figure 3. Inhibition of Stably Expressed Firefly Luciferase Using the TransIT-siQUEST® Reagent. Cell lines stably expressing firefly luciferase were transfected with an anti-firefly luciferase siRNA or a reagent alone control using the TransIT-siQUEST® Reagent. Twenty-four hours post-transfection, luciferase expression was measured and compared to the reagent alone control.
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Figure 4:Efficient Target Gene Knockdown in Selected Cell Lines Using TransIT-siQUEST Reagent.
Figure 4. Efficient Target Gene Knockdown in Selected Cell Lines Using TransIT-siQUEST® Reagent. Reporter plasmids expressing both firefly and sea pansy luciferase were co-transfected into the indicated cell lines using TransIT® Plasmid Transfection Reagents. Targeted knockdown was achieved by transfection of an anti-firefly luciferase siRNA using the TransIT-siQUEST® Reagent. Twenty-four hours later, firefly luciferase expression was normalized to sea pansy luciferase expression and compared to the reagent alone control.
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TransIT siQUEST Hela TransIT siQUEST Reagent Alone
A B
Figure 5. Visualization of Fluorescently-Labeled siRNA Transfected with the TransIT-siQUEST® Reagent. (A) HeLa cells were transfected with a Cy™3-labeled siRNA using the TransIT-siQUEST® Reagent or (B) a reagent alone control. At 4 hours post-transfection, the cells were fixed, counterstained, and analyzed by confocal microscopy to visualize the siRNA (red), nuclei (blue), and actin (green).
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