TransIT®-mRNA Transfection Kit

A high efficiency, low toxicity, large RNA transfection reagent for mammalian cells

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• Low Cellular Toxicity - Maintain cell density and reduce experimental biases.
• High Efficiency Delivery - Achieve RNA delivery in a large population of cells to ensure experimental success.
• Serum Compatible - Perform transfections in the presence of serum which eliminates the need for a media change and maintains cellular health.
• Deliver Various Sizes of RNA - Ideal for specialized applications, such as viral production and protein expression from mRNA.

Data

Figure 1: The TransIT®-mRNA Transfection Kit Transfects GFP mRNA into DC 2.4 Dendritic Cells
Figure 2: Multiple Dendritic Cell Types Express GFP from mRNA Transfected by TransIT®-mRNA Transfection Kitfection Kit
Figure 3: High Level Luciferase Expression after Delivery of a Luciferase mRNA using the TransIT®-mRNA Transfection Kit.
Figure 4: The TransIT®-mRNA Transfection Kit Efficiently Delivers the LacZ mRNA to CHO-K1 Cells.

A	B	C

Figure 1. The TransIT®-mRNA Transfection Kit Transfects GFP mRNA into DC 2.4 Dendritic Cells. Using TransIT-mRNA Transfection Kit, DC 2.4 cells were transfected with (A) 0.5 µg, (B) 1 µg and (C) 2.5 µg of capped and polyadenylated mRNA encoding GFP with 1 µl TransIT-mRNA Reagent and 1 µl Boost. Cells were seeded overnight at 100,000 cells/well in 24-well plates.  Images were taken 10 hours post-transfection.

Data courtesy of Kyle Phua (Principal Investigator: Kam W. Leong), Duke University

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Figure 2. Multiple Dendritic Cell Types Express GFP from mRNA Transfected by TransIT®-mRNA Transfection Kit. Murine primary bone marrow derived dendritic cells (BMDC) and murine dendritic cells types (JAWSII and DC 2.4) were transfected with 1 µg of capped and polyadenlyated mRNA encoding GFP using a TransIT-mRNA Reagent: Boost: mRNA ratio of 1:1:1 (µl:µl:µg). Primary BMDCs, JAWSII and DC 2.4 were seeded (80,000 cell/well) overnight in 24-well plates. Cells were assayed via flow cytometry 8 hours post transfection. Error bars represent the standard deviation of at least 3 separate experiments.

Data courtesy of Kyle Phua (Principal Investigator: Kam W. Leong), Duke University

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Figure 3. High Level Luciferase Expression after Delivery of a Luciferase mRNA using the TransIT®-mRNA Transfection Kit. Cells in 12-well plates were transfected with a capped and polyadenylated mRNA encoding luciferase using the TransIT-mRNA Transfection Kit. Approximately 18 hrs post-transfection the cells were harvested and the total luciferase activity per well was determined.

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A. Mock Transfection Control	B. LacZ mRNA Transfection

Figure 4. The TransIT®-mRNA Transfection Kit Efficiently Delivers LacZ mRNA to CHO-K1 Cells. Using the TransIT-mRNA Transfection Kit, CHO-K1 cells were mock transfected (A) or transfected with a capped and polyadenylated LacZ encoding mRNA (B). Approximately 18 hrs post-transfection the cells were stained using Mirus Bio’s Beta-gal Staining Kit to identify the LacZ transfected cells.

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