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TransIT®-mRNA Transfection Kit

A high efficiency, low toxicity, large RNA transfection reagent for mammalian cells

Product No.QuantityPriceAdd to Cart
TransIT®-mRNA Transfection Kit
MIR 22250.4 ml$317.00
MIR 22501.0 ml$567.00
MIR 22555 x 1.0 ml$2,491.00
MIR 225610 x 1.0 ml$4,611.00
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.

Resources

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Poster: Highly Efficient Delivery of Viral RNA...
Article: Selection of an RNA transfection reagent...
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Each Kit is supplied with the TransIT-mRNA Transfection Reagent and the mRNA Boost Reagent.

  • Low Cellular Toxicity - Maintain cell density and reduce experimental biases.
  • High Efficiency Delivery - Achieve RNA delivery in a large population of cells to ensure experimental success.
  • Serum Compatible - Perform transfections in the presence of serum which eliminates the need for a media change and maintains cellular health.
  • Deliver Various Sizes of RNA - Ideal for specialized applications, such as viral production and protein expression from mRNA.
 

Recent Advances in Stem Cell Technology Utilize mRNA Transfection to Generate Induced Pluripotent Stem Cells.

Find out more in the following articles.

Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Warren et al. 2010. Cell Stem Cell. Volume 7, Issue 5, 618-630.

Innate Immune Suppression Enables Frequent Transfection with RNA Encoding Reprogramming Proteins. Angel M, Yanik MF. 2010. PLoS One. Jul 23;5(7):e11756.
 

Customer Testimonial

Dr. Katalin Kariko
University of Pennsylvania - Department of Neurosurgery

“Our lab recently used the TransIT®-mRNA Transfection Kit to show that intracellular delivery of HPLC-purified and pseudouridine-containing mRNA can translate very efficiently without immune activation which is ideal for mRNA-based gene therapy applications. TransIT®-mRNA further facilitated this work through low toxicity transfections of HEK 293T, human dendritic cells (DCs) and primary keratinocytes (Karikó et al. Nucleic Acids Research, 39:e142, 2011).”

 

Additional Information

TransIT-mRNA for high-titer virus production
TransIT-mRNA Comparison Data to Electroporation and TransMessenger™ Reagent
Cell lines successfully transfected at Mirus using TransIT-mRNA Transfection Kit (pdf)

Data

Figure 1: The TransIT®-mRNA Transfection Kit Transfects GFP mRNA into DC 2.4 Dendritic Cells
Figure 2: Multiple Dendritic Cell Types Express GFP from mRNA Transfected by TransIT®-mRNA Transfection Kitfection Kit
Figure 3: High Level Luciferase Expression after Delivery of a Luciferase mRNA using the TransIT®-mRNA Transfection Kit.
Figure 4: The TransIT®-mRNA Transfection Kit Efficiently Delivers the LacZ mRNA to CHO-K1 Cells.

TransIT mRNA Transfects GFP mRNA, Figure A TransIT mRNA Transfects GFP mRNA, Figure B TransIT mRNA Transfects GFP mRNA, Figure C
A B C
Figure 1. The TransIT®-mRNA Transfection Kit Transfects GFP mRNA into DC 2.4 Dendritic Cells. Using TransIT-mRNA Transfection Kit, DC 2.4 cells were transfected with (A) 0.5 µg, (B) 1 µg and (C) 2.5 µg of capped and polyadenylated mRNA encoding GFP with 1 µl TransIT-mRNA Reagent and 1 µl Boost. Cells were seeded overnight at 100,000 cells/well in 24-well plates.  Images were taken 10 hours post-transfection.

Data courtesy of Kyle Phua (Principal Investigator: Kam W. Leong), Duke University
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Figure 2:Multiple Dendritic Cell Types Express GFP from mRNA Transfected by TransIT®-mRNA Transfection Kitfection Kit
Figure 2. Multiple Dendritic Cell Types Express GFP from mRNA Transfected by TransIT®-mRNA Transfection Kit. Murine primary bone marrow derived dendritic cells (BMDC) and murine dendritic cells types (JAWSII and DC 2.4) were transfected with 1 µg of capped and polyadenlyated mRNA encoding GFP using a TransIT-mRNA Reagent: Boost: mRNA ratio of 1:1:1 (µl:µl:µg). Primary BMDCs, JAWSII and DC 2.4 were seeded (80,000 cell/well) overnight in 24-well plates. Cells were assayed via flow cytometry 8 hours post transfection. Error bars represent the standard deviation of at least 3 separate experiments.

Data courtesy of Kyle Phua (Principal Investigator: Kam W. Leong), Duke University
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Figure 3:High Level Luciferase Expression after Delivery of a Luciferase mRNA using the TransIT®-mRNA Transfection Kit.
Figure 3. High Level Luciferase Expression after Delivery of a Luciferase mRNA using the TransIT®-mRNA Transfection Kit. Cells in 12-well plates were transfected with a capped and polyadenylated mRNA encoding luciferase using the TransIT-mRNA Transfection Kit. Approximately 18 hrs post-transfection the cells were harvested and the total luciferase activity per well was determined.
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TransIT mRNA Mock Transfection TransIT mRNA lacZ
A. Mock Transfection Control B. LacZ mRNA Transfection
Figure 4. The TransIT®-mRNA Transfection Kit Efficiently Delivers LacZ mRNA to CHO-K1 Cells. Using the TransIT-mRNA Transfection Kit, CHO-K1 cells were mock transfected (A) or transfected with a capped and polyadenylated LacZ encoding mRNA (B). Approximately 18 hrs post-transfection the cells were stained using Mirus Bio’s Beta-gal Staining Kit to identify the LacZ transfected cells.
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