TransIT®-LT1 Transfection Reagent

Figure 1. High Efficiency Transfection of iCell® Cardiomyocytes using TransIT®-LT1 Transfection Reagent. GFP transiently transfected into iCell Cardiomyocytes verifies relatively high transfection efficiencies. See more information on stem cell applications.

Data courtesy of

Figure 2. The TransIT®-LT1 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents. HepG2 cells were transfected with a luciferase expression plasmid using the designated reagents at the manufacturer’s recommended reagent-to-DNA ratio indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph. FuGENE is a registered trademark of Fugent LLC. Lipofectamine is a trademark of Life Technologies Corporation.

Figure 3. Comparable Luciferase Expression with the TransIT® -LT1 Reagent and FuGENE® 6 in Multiple Cell Types. The indicated cell lines were transfected in duplicate with 1 µg of a luciferase expression vector per well of a 12-well plate using either 3 µl of the TransIT®-LT1 or FuGENE® 6 Reagents. Cells were harvested 24 hours post-transfection and assayed for luciferase activity. FuGENE is a registered trademark of Fugent LLC.

Figure 4. The TransIT®-LT1 Reagent Efficiently Delivers DNA to a Wide Variety of Cell Lines. Using the TransIT®-LT1 Transfection Reagent, cells were transfected with the pEGFP-C1 expression vector, and the percentage of EGFP expressing cells was determined 24-48 hours post-transfection.