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TransIT®-LT1 Transfection Reagent

A broad spectrum, low toxicity, DNA transfection reagent

Product No.QuantityPriceAdd to Cart
TransIT®-LT1 Transfection Reagent
MIR 23040.4 ml$175.00
MIR 23001 ml$276.00
MIR 23055 x 1 ml$1,205.00
MIR 230610 x 1 ml$2,229.00
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.

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  • Broad Spectrum DNA Delivery - Utilize one transfection reagent and protocol for a variety of cells.
  • Low Cellular Toxicity - Maintain cell density and reduce experimental biases.
  • High Efficiency Delivery - Achieve expression in a large population of cells for experimental success.
  • Deliver Single or Multiple Plasmids - Suitable for many applications such as gene expression, shRNA expression, viral production, and promoter analysis.

TransIT®-LT1 exhibits lower toxicity than Lipofectamine™ 2000 and Lipofectamine LTX with Plus™ Reagents
TransIT-LT1 for high-titer virus production
 

Customer Testimonial

Beverly Wuertz
University of Minnesota - F. Ondrey Lab
"Our lab regularly transfects head and neck cancer squamous carcinoma cell lines as well as pre-cancerous oral lesion cell lines and primary cells with Mirus Bio products. We recently published a paper using Mirus Bio TransIT-LT1 in which we concluded tobacco carcinogens up-regulate AP-1 activity and AP-1 dependent IL-8 and VEGF gene expression in head and neck cancer." (Swenson, W et al. Molecular Carcinogenesis. April 2011)

Data

Figure 1: Higher Expression and Lower Toxicity with TransIT-LT1
Figure 2: The TransIT-LT1 Reagent Efficiently Delivers DNA to a Variety of Cell Lines.
Figure 3: The TransIT-LT1 Reagent Performs Best in the Presence of Serum Containing Media.

Figure 1:Higher Expression and Lower Toxicity with TransIT-LT1
Figure 1. The TransIT®-LT1 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents. HepG2 cells were transfected with a luciferase expression plasmid using the designated reagents at the manufacturer’s recommended reagent-to-DNA ratio indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph.
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Figure 2:The TransIT-LT1 Reagent Efficiently Delivers DNA to a Variety of Cell Lines.
Figure 2. The TransIT®-LT1 Reagent Efficiently Delivers DNA to a Wide Variety of Cell Lines. Using the TransIT®-LT1 Transfection Reagent, cells were transfected with the pEGFP-C1 expression vector, and the percentage of EGFP expressing cells was determined 24-48 hours post-transfection.
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Figure 3:The TransIT-LT1 Reagent Performs Best in the Presence of Serum Containing Media.
Figure 3. The TransIT®-LT1 Reagent Performs Best in the Presence of Serum Containing Media. Using the TransIT®-LT1 Reagent, the indicated cells lines were transfected with a luciferase expression vector using three different media conditions: cells were transfected in the presence of (1) serum-free media or (2) complete media with media changes 4 hours post-transfection, or transfected in the presence of (3) complete media with no media change post-transfection. The cells were harvested 48 hours post-transfection, and the total luciferase activity per well was determined.
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