Shopping Cart - 0 Items, $0   View | Checkout

TransIT®-2020 Transfection Reagent

A high performance, animal-free, broad spectrum DNA transfection reagent

Product No.QuantityPriceAdd to Cart
TransIT®-2020 Transfection Reagent
MIR 54040.4 ml$188.00
MIR 54001 ml$375.00
MIR 54055 x 1 ml$1,642.00
MIR 540610 x 1 ml$3,037.00
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.

Resources

Protocol
MSDS
Frequently Asked Questions
Product Citations
Tips From the Bench
Contact Technical Support
Request a Sample

Reagent Agent Don't See Your Cell Type? Ask Reagent Agent

View Data
 
 
  • Broad Spectrum DNA Delivery - Achieve high expression in many cell types, including hard to transfect cell lines and primary cells
  • Outperforms Competitor Reagents - TransIT®-2020 demonstrated higher protein yield when compared to FuGENE® HD, Lipofectamine™ 2000, and Lipofectamine™ 2000 CD
  • Superior Transfection of Insect Cells - Obtain higher expression than other insect cell transfection reagents
  • Animal Origin Free - TransIT®-2020 provides high performance with maximum compatibility

Cell lines successfully transfected using TransIT-2020 (pdf)
Primary or stem cells successfully transfected using TransIT-2020 (pdf)
Insect cell lines successfuly transfected using TransIT-2020 (pdf)
TransIT-2020 for high-titer virus production
 

Customer Testimonial

John A. Burns
David Scicchitano Lab, New York University – Department of Biology
“Our lab routinely works with primary human fibroblasts which are notoriously difficult to transfect, and we require a method that is non-toxic, reliable, and reproducible. TransIT-2020 is the only reagent on the market that fits those criteria. In addition, TransIT-2020 also transfects colon cancer cell lines HCT-116 and DLD1 with much higher efficiencies. Overall, the pace and quality of our research have improved since we started using TransIT-2020.”

Data

Figure 1: TransIT-2020 Reagent Exhibits Higher Expression and Lower Cell Toxicity Compared to Other Transfection Reagents
Figure 2: High Performance Plasmid Transfection
Figure 3: Superior Gene Expression in a Broad Spectrum of Cell Types
Figure 4: TransIT®-2020 Reagent Effectively Transfects Drosophila S2 Cells

Figure 1:TransIT-2020 Reagent Exhibits Higher Expression and Lower Cell Toxicity Compared to Other Transfection Reagents
Figure 1. TransIT®-2020 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents. Human umbilical vein endothelial cells (HUVEC) were transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph. Error bars represent the standard deviation of triplicate wells.
Back to Top
 
Figure 2:High Performance Plasmid Transfection
Figure 2. High Performance Plasmid Transfection. Primary Human Small Epithelial Cells (HSAEpic) were transfected using TransIT-2020 and an EGFP expression plasmid (4:1 reagent-to-DNA ratio). Images were taken 24 hours post-transfection using a Zeiss axiovert inverted fluorescence microscope.
Back to Top
 
Figure 3:Superior Gene Expression in a Broad Spectrum of Cell Types
Figure 3. Superior Gene Expression in a Broad Spectrum of Cell Types. The indicated cell types were transfected in 96-well plates with a luciferase expression plasmid (0.1ug/well) according to the manufacturer’s protocol. Reagent to DNA ratios were optimized for each cell type:  TransIT®-2020 (Mirus Bio, 2:1 or 3:1), FuGENE® HD (Roche, 3.5:1), Lipofectamine™ 2000 (Life Technologies, 1.5:1, 3:1 or 5:1).  Luciferase activity was measured 24 hours post-transfection. Values were normalized to TransIT-2020 and presented as a percentage of luciferase expression.
Back to Top
 
Figure 4:TransIT®-2020 Reagent Effectively Transfects Drosophila S2 Cells
Figure 4. TransIT®-2020 Reagent Effectively Transfects Drosophila S2 Cells. Cells were transfected with a plasmid construct expressing a secreted form of the Dscam extracellular domain fused to alkaline phosphatase (AP) in a 24-well plate. The ratio of transfection reagent to DNA and micrograms of DNA per well is noted beneath each bar. All products were used according to manufacturers’ protocol. All transfections were performed in serum-free media for four hours followed by complete media supplementation. An AP enzymatic assay was used to measure the AP levels 24 hours post-transfection.

Data courtesy of Woj Wojtowicz, University of California, Berkeley.
Back to Top