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NEW! 3D Transfection System

High efficiency broad spectrum DNA transfection of cells in 3D culture for biologically relevant data

Product No.QuantityPriceAdd to Cart
3D Transfection System
MIR 58200.4 ml TransIT®-3D Transfection Reagent & 2 x 12-well alvetex® plates$300.00
TransIT®-3D Transfection Reagent
MIR 58040.4 ml$196.00
MIR 58001 ml$300.00
MIR 58055 x 1 ml$1,313.00
MIR 580610 x 1 ml$2,430.00
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.
To order additional 12-well alvetex® plates,
contact Reinnervate.
Reinnervate Alvetex

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Ideal for the transfection of cells in 3D cell culture, the new 3D Transfection System combines the technologies of Reinnervate's alvetex® 3D Cell Culture Plates and Mirus Bio's TransIT®-3D Transfection Reagent, enabling scientists to create models that more accurately mimic the tissue environment, gaining a much deeper insight into the complexities of cell function and behavior.

High Efficiency DNA Delivery - Achieve high expression in a wide range of cell types grown in 3D culture
Easy-to-Use - User friendly protocol that requires minimal optimization
Flexibility - Compatible with solid matrix scaffold and hydrogel 3D culture formats
Animal Origin Free - Allows a wide range of applications

Data

Figure 1: Alvetex® Scaffold for 3D Cell Culture
Figure 2: 3D Transient Transfection Protocol
Figure 3: GFP Expression in 3D Culture
Figure 4: 3D Transfection of Multiple Cell Types

  A. Alvetex 12-well Plates for 3D Cell Culture B. Alvetex Scaffold C. TransIT 3D Alvetex hacat cells  
Figure 1. Alvetex® Scaffold for 3D Cell Culture. (A) Presentation of alvetex in a 12-well plate format. (B) Alvetex is a highly porous cross-linked polystyrene scaffold, which as been sectioned into 200 µm thick membranes. (C) Cross section of HaCaT cells grown for 3 days in submerged media conditions and 14 days at the air interface.
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Figure 2:3D Transient Transfection Protocol
Figure 2. 3D Transient Transfection Protocol. Using the 3D Transfection System, cells are quickly adapted to 3D culture in alvetex® 12-well plates. Post-adaptation, the cells are transfected with high efficiency and minimal optimization using the TransIT®-3D Transfection Reagent.
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Figure 3:GFP Expression in 3D Culture

Figure 3. GFP Expression in 3D Culture. NIH3T3 fibroblast cells were seeded at optimized cell density in alvetex® 12-well plates and adapted to 3D growth. Forty-eight hours post-adaptation, cells were transfected with TransIT®-3D combined with a plasmid encoding Green Fluorescent Protein (GFP). Cells were counterstained with the nuclear stain Hoechst 33342 (blue) and visualized via confocal microscopy.

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Figure 4:3D Transfection of Multiple Cell Types
Figure 4. 3D Transfection of Multiple Cell Types. The indicated cell lines were seeded at optimized cell densities in 12-well alvetex® 3D plates and adapted to 3D culture conditions for 48 hours. Post-adaptation, cells were transfected with TransIT®-3D combined with a plasmid encoding firefly luciferase at the reagent-to-DNA ratios indicated. Luciferase activity was measured 24 hours post-transfection using a conventional assay.
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