Image Strip

Label IT® Tracker™ Intracellular Nucleic Acid Localization Kits

Efficient, direct labeling of plasmid DNA for in vitro and in vivo tracking experiments

Product No.QuantityPriceAdd to
Cy™3 Labeling Kit
MIR 7010labels 50-200 µg plasmid DNA$302.00
Cy™5 Labeling Kit
MIR 7011labels 50-200 µg plasmid DNA$302.00
Fluorescein Labeling Kit
MIR 7015labels 50-200 µg plasmid DNA$269.00
CX-Rhodamine Labeling Kit
MIR 7012labels 50-200 µg plasmid DNA$269.00
TM-Rhodamine Labeling Kit
MIR 7013labels 50-200 µg plasmid DNA$269.00
Biotin Labeling Kit
MIR 7014labels 50-200 µg plasmid DNA$269.00
NOTE: Each kit contains sufficient reagents to perform at least 50 transfections in 6-well plates. Each kit also contains 0.4 ml of TransIT®-LT1 Reagent, our broad spectrum in vitro transfection reagent.
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.
 

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  • Versatile Labeling—Efficiently label and visualize the DNA of your choice.
  • One-step Chemical Method—Easily and consistently control the labeling reactions.
  • High Efficiency Labeling—Optimal visualization of DNA in cells.
  • Superior Tracking—Monitor both subcellular localization and reporter transgene expression following transfection of labeled (plasmid) DNA.

Kit Components:

Each Kit contains Label IT Tracker Reagent, TransIT-LT1 Reagent, Label IT Reconstitution Solution, and 10X Labeling Buffer.

Data

Figure 1A3 Hours Figure 1B8 Hours Figure 1C20 Hours
 
Figure 1. Tracking of Plasmid Localization and Expression. COS-7 cells transfected with Label IT Tracker Cy™5 (blue) labeled pEYFP-nuc and TransIT-LT1 Reagent in serum containing media. Images were acquired at 3, 8, and 20 hours post-transfection. The blue signal indicates the cellular localization of the labeled plasmid, the green signal indicates cellular autofluorescence, and the yellow signal is the expression of the nuclear yellow fluorescent protein (YFP) reporter.
Figure 2 Figure 2. Tracking of Plasmid Localization. COS-7 cells were transfected with Label IT Tracker Biotin labeled pEYFP-nuc and TransIT-LT1 Reagent in serum containing media. Image was acquired at 24 hours post-transfection after staining with streptavidin Cy™3 (red) conjugate. The green signal indicates cellular autofluorescence.

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