Label IT® siRNA Tracker Intracellular Localization Kits
Efficient, direct labeling for in vitro and in vivo tracking of siRNA
| Product No. | Quantity | Price | Add to |
| Cy™3 Labeling Kit |
| MIR 7212 | labels 50 µg siRNA | $320.00 |
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| Cy™5 Labeling Kit |
| MIR 7213 | labels 50 µg siRNA | $320.00 |
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| Fluorescein Labeling Kit |
| MIR 7216 | labels 50 µg siRNA | $291.00 |
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| CX-Rhodamine Labeling Kit |
| MIR 7214 | labels 50 µg siRNA | $291.00 |
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| TM-Rhodamine Labeling Kit |
| MIR 7215 | labels 50 µg siRNA | $291.00 |
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| Biotin Labeling Labeling Kit |
| MIR 7217 | labels 50 µg siRNA | $291.00 |
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NOTE: Each kit contains sufficient quantities to label 50 µg siRNA.
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- Versatile Labeling—Efficiently label and visualize the siRNA of your choice.
- One-step Chemical Method—Easily and consistently control the labeling reactions and labeling density.
- High Efficiency Labeling—Optimal visualization of siRNA in cells and tissues.
Kit Components:
Each Kit contains Label IT® siRNA Tracker Reagent, Label IT® Reconstitution Solution, 10X Labeling Buffer A , and siRNA Dilution Buffer.
For more information on siRNA delivery in vivo, see the following references:
Lewis et al (2002) Nature Genetics 32: 107-107.
Zhang et al (1999) Human Gene Therapy 10: 1735-1737.
Liu et al (1999) Gene Therapy 6: 1258-1266.
Hagstrom et al (2004) Molecular Therapy 10: 386-398.
Data
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| Figure 1A |
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Figure 1B |
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| Figure 1. Delivery of Labeled siRNA to Mouse Liver. siRNA (25 µg) was labeled with Label IT® siRNA Tracker Cy™3 Kit and delivered to mice through the tail vein using either low volume injection conditions (100 µl)(Figure 1A) or hydrodynamic injection conditions (2 ml over 6-8 seconds) (Figure 1B). Mouse livers were harvested 30 minutes after injection, fixed in paraformaldehyde, sectioned (10 µm thick) and analyzed. A representative hepatocyte (h) and sinusoid (s) are indicated in each panel. The hydrodynamic injection of siRNA results in siRNA uptake by the majority of the hepatocytes in the liver. |
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