Label IT® Nucleic Acid Modifying Reagents
Efficient, direct, non-enzymatic attachment of functional groups to DNA and RNA
| Product No. | Quantity | Price | Add to |
| Amine Modifying Kit |
| MIR 3925 | * | $199.00 |
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| MIR 3900 | ** | $499.00 |
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| COOH Modifying Kit |
| MIR 4025 | * | $199.00 |
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| MIR 4000 | ** | $499.00 |
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* The trial size kit provides sufficient reagents for modifying 25 µg of nucleic acid.
** The full size kit provides sufficient reagents for modifying 100 µg of nucleic acid.
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Resources
Protocol
MSDS
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- Modify Any DNA or RNA Template—Direct, covalent attachment of amine or carboxylic acid functional groups to any nucleic acid.
- One-step Chemical Method—Easily and consistently control the density of nucleic acid modification.
- Covalent Mechanism—Permanent, non-destructive modification of nucleic acids which can then be conjugated to proteins or peptides, or attached to glass surfaces, or beads or plates.
Kit Components:
Each Kit contains Label IT Reagent, Label IT Reconstitution Solution, Labeling Buffer, Solutions and G50 Spin Columns.
Data
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| Figure 1. Demonstration of the Functionality of Amine Groups on Modified DNA. Plasmid DNA (5.6 kb) was reacted with Label IT Amine Reagent at the ratios (w:w) of labeling reagent to DNA indicated and purified by ethanol precipitation. Five µg of the modified DNA, in triplicate, was reacted with 10 mM NHS ester TM-Rhodamine and 100 mM NaHCO3 (pH 8 - 8.5) for 1 hour at room temperature in the dark. The DNA was again ethanol precipitated, washed extensively, and assayed for labeling efficiency. |
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| Figure 2. Demonstration of the Functionality of Carboxylic Acid Groups on Modified DNA. Plasmid DNA (5.6 kb) was reacted with Label IT COOH reagent at the ratios (w:w) of labeling reagent to DNA indicated and purified by ethanol precipitation. Ten µg of the modified DNA, in triplicate, was reacted with 1 mg/ml EDC (1-ethyl-3-(-dimethylaminopropyl carbodiimide HCl), freshly prepared in 25 mM MES (pH 6-6.5) and 1 mg/ml Lissamine™ Rhodamine B ethylenediamine for 3 h at room temperature, protected from light. The DNA was again ethanol precipitated, washed extensively and assayed for labeling efficiency (determined by Rhodamine fluorescence intensity (FI) per µg of recovered DNA). |
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