HybQUEST® Hybridization and Detection Kits
Efficient, direct, non-enzymatic labeling of DNA probes for membrane hybridizations
| Product No. | Quantity | Price | Add to |
| Complete DNP System |
| MIR 6000 | 5 x 2 µg rxns/Kit (Enough to process ≥1000 cm2 membrane) | $575.00 |
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| Hybridization and Detection (DNP) Kit |
| MIR 6010 | 10 blots | $377.00 |
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| Label IT® (Fluorescein) Kit |
| MIR 6200 | 5 x 2 µg rxns* | $225.00 |
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| Label IT® (Digoxin) Kit |
| MIR 6300 | 5 x 2 µg rxns* | $225.00 |
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| Label IT® (Biotin) Kit |
| MIR 6400 | 5 x 2 µg rxns* | $225.00 |
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| Label IT® (DNP) Kit |
| MIR 6800 | 5 x 2 µg rxns* | $225.00 |
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Resources
Protocols
MSDS
Contact Technical Support
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- Optimized Protocol—Detailed instructions and validation for slot blot, colony lift, and genomic southern hybridizations.
- Direct and Indirect Sensitive Detection—Achieve high sensitivity (sub-picogram) hybridization detection.
- One-step Chemical Method—Easily and consistently control the labeling reaction.
- Covalent Mechanism—Permanent, non-destructive modification of nucleic acid residues is ideal for hybridization; labels do not impact hybridization performance.
Kit Components:
Each FISH Kit includes the appropriate Cy™3, TM-Rhodamine, Fluorescein, or Biotin labeling reagent, solutions and hybridization buffer. Please note that the Label IT® FISH Biotin Kit does not contain Biotin detection reagents.
Data
Figure 1: Slot Blot Hybridization.
Figure 2: Colony Lift Hybridization.
Figure 3: Genomic Southern Hybridization-GAPDH.
Figure 4: Simple Southern Hybridization.
Figure 5: Genomic Southern Hybridization- Human Factor IX.
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Figure 1. Slot Blot Hybridization. A DNP-labeled probe, prepared using the HybQUEST System, was used to detect decreasing amounts of target DNA in a slot blot application. Starting at 100 ng, ten-fold serial dilutions of denatured DNA were blotted onto a membrane in decreasing concentrations from top to bottom. The hybridization and detection of the DNP-labeled DNA was performed using the HybQUEST Complete Kit. The data show that as little as 0.1 pg of DNA can be detected. |
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Figure 2. Colony Lift Hybridization. A segment of a bacterial colony lift membrane, prepared from E. coli DH10b/pCI-luciferase, was hybridized with a DNP-labeled luciferase probe according to the HybQUEST protocol. |
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Figure 3. Genomic Southern Hybridization. Analysis of human genomic DNA (5 µg, EcoR1-digested) using a 900 bp DNP-labeled human GAPDH probe and the HybQUEST Complete (DNP) System. GAPDH has been characterized as a single expressed locus with one pseudogene and 15 related loci. |
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Figure 4. Simple Southern Hybridization. Alongside a resolved DNP-labeled DNA ladder (lane 1), 10 pg of target DNA (lane 2), and 10 pg of non-target DNA (lane 3) were blotted to a nylon membrane. A DNP-labeled probe (homologous to the target DNA in lane 2) was used in the hybridization analysis according to the HybQUEST recommended protocol. |
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Figure 5. Genomic Southern Hybridization. Detection of the single copy gene (human Factor IX) in 10 µg (lane 2) and 5 µg (lane 3) of EcoRI-digested human genomic DNA using a 1.4 kb DNP-labeled cDNA probe. Lane 1 is a DNP-labeled 1 kb ladder. |
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