Label IT® µArray® Labeling Kits, Cy™3 and/or Cy™5
Optimized labeling kit designed for dual-channel microarray applications using Cy™3 and/or Cy™5 dyes
- One-step Chemical Method—Easily and consistently achieve optimized labeling densities for microarray applications.
- Sensitive Detection—Confidently detect rare transcripts and small changes in gene expression.
- Versatile Labeling—Suitable for labeling mRNA, cDNA and cRNA templates with Cy™3 and Cy™5 dyes for expression profiling analysis.
Kit Components:
Each Kit contains Label IT Cy™3 and/or Cy™5 Reagents, Reconstitution Solution, 10X Labeling Buffer M, Reagent D1, Buffer N1, 0.5M EDTA, 10X STOP Reagent, and 5X Fragmentation Buffer.
Data
Figure 1: Direct Labeling and Hybridization of mRNA Samples Using the Label IT µArray Cy™3/Cy™5 Kit.
Figure 2: Direct mRNA Labeling for Gene Expression Profile Analysis.
Figure 3: Array Based Comparative Genomic Hybridization Applications Using the Label IT µArray Cy™3/Cy™5 Kit.
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Figure 1. Direct Labeling and Hybridization of mRNA Samples Using the Label IT µArray® Labeling Kit, Cy™3/Cy™5. RNA samples were enriched for mRNA, labeled with the Label IT µArray® Labeling Kit, Cy™3/Cy™5, pooled, and hybridized to a cDNA IntelliGene™ TestARRAY v4.0 (TaKaRa Mirus Bio Inc.). |
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| Figure 2. Direct mRNA Labeling for Gene Expression Profile Analysis. HeLa and A549 mRNA samples were labeled in a “dye-swap” experiment comparing the Label IT µArray® Labeling Kit, Cy™3/Cy™5 to enzymatic incorporation of Cy™3 and Cy™5 (SuperScript™ Direct cDNA Labeling System; Invitrogen). In this “dye-swap” experiment, the average log2(Cy™3/Cy™5) signal ratio for each gene is expected to change sign (e.g. from +1 to -1) when samples are labeled for detection in the opposite channel. |
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| Figure 3. Array Based Comparative Genomic Hybridization Applications Using the Label IT µArray® Labeling Kit, Cy™3/Cy™5. Mse I/Alu I digested human male and female DNA samples were labeled with Cy™5 and Cy™3, respectively, using the kit. 2.5 µg of each sample was hybridized to a BAC array (Spectral Genomics), according to the recommended procedure for CGH analysis. The red features indicate signal from the male sample; green features indicate signal from the female sample. |
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