Label IT® siRNA Tracker Intracellular Localization Kits, without Transfection Reagent

Figure 1. The Label IT® siRNA Tracker Kits. The Label IT siRNA Tracker labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with the siRNA and miRNA and the reactive alkylating group (blue) that covalently attaches the Label IT reagents to any reactive heteroatom within the siRNA or miRNA. Attachment of the Label IT Reagent to siRNA or miRNA does not alter their structure or affect downstream target knockdown performance, and as such, the same labeled siRNAs or miRNAs can be both visualized after transfection by fluorescence microscopy and employed to knockdown target gene expression.

Figure 2. Delivery of Labeled siRNA to Mouse Liver. siRNA (25 µg) was labeled with Label IT® siRNA Tracker Cy™3 Kit and delivered to mice through the tail vein using either low volume injection conditions (100 µl)(Figure 2A) or hydrodynamic injection conditions (2 ml over 6-8 seconds) (Figure 2B). Mouse livers were harvested 30 minutes after injection, fixed in paraformaldehyde, sectioned (10 µm thick) and analyzed. A representative hepatocyte (h) and sinusoid (s) are indicated in each panel. The hydrodynamic injection of siRNA results in siRNA uptake by the majority of the hepatocytes in the liver.