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Label IT® Nucleic Acid Labeling Reagents

Efficient, direct, non-enzymatic labeling of DNA and RNA

Product No.QuantityPriceAdd to
Label IT® Nucleic Acid Labeling Kit, Biotin
MIR 3425labels 25 µg nucleic acid $181.00
MIR 3400labels 100 µg nucleic acid $457.00
Label IT® Nucleic Acid Labeling Kit, CX-Rhodamine
MIR 3125labels 25 µg nucleic acid $181.00
MIR 3100labels 100 µg nucleic acid $457.00
Label IT® Nucleic Acid Labeling Kit, Cy™3
MIR 3625labels 25 µg nucleic acid $216.00
MIR 3600labels 100 µg nucleic acid $540.00
Label IT® Nucleic Acid Labeling Kit, Cy™5
MIR 3725labels 25 µg nucleic acid $216.00
MIR 3700labels 100 µg nucleic acid $540.00
Label IT® Nucleic Acid Labeling Kit, Digoxin
MIR 3325labels 25 µg nucleic acid $181.00
MIR 3300labels 100 µg nucleic acid $457.00
Label IT® Nucleic Acid Labeling Kit, DNP
MIR 3825labels 25 µg nucleic acid $181.00
MIR 3800labels 100 µg nucleic acid $457.00
Label IT® Nucleic Acid Labeling Kit, Fluorescein
MIR 3225labels 25 µg nucleic acid $181.00
MIR 3200labels 100 µg nucleic acid $457.00
Label IT® Nucleic Acid Labeling Kit, TM-Rhodamine
MIR 4125labels 25 µg nucleic acid $181.00
MIR 4100labels 100 µg nucleic acid $457.00
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.

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View Data
  • Label Any DNA or RNA Template - Suitable for a wide range of applications.
  • One-step Chemical Method - Easily and consistently control the labeling reactions.
  • Adjustable Labeling Density - Achieve high sensitivity with optimally labeled DNA and RNA.
  • Covalent Mechanism - Permanent, non-destructive modification of nucleic acid residues is ideal for many diverse applications; labels do not impact hybridization performance.

Kit Components:
Each Kit contains a Label IT Reagent, Label IT Reconstitution Solution, 10X Labeling Buffer and solutions.

Data

Figure 1: The Label IT Nucleic Acid Labeling Kits.
Figure 2: Non-destructive Direct Labeling of RNA.
Figure 3: Generation of Sensitive FISH Probes.
Figure 4: Labeling Density can be Easily Controlled with the Label IT Reagents.
Figure 5: Excitation and Emission Wavelengths of Fluorescent Label IT Reagents.

Figure 1
Figure 1. The Label IT® Nucleic Acid Labeling Kits. The Label IT chemical labeling reagents are composed of three regions: the label (fluorophore or hapten) (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT reagent to any reactive heteroatom within the nucleic acids. Attachment of the Label IT Reagents to nucleic acids does not alter the structure of the nucleic acid or affect downstream hybridization performance, and as such, nucleic acids labeled using the Label IT Reagents can be employed in multiple applications as defined by the researcher.
Figure 2
Figure 2. Non-destructive Direct Labeling of RNA. Mouse total RNA was labeled with the Label IT® Fluorescein Reagent and resolved, alongside unlabeled total RNA, on a 1% agarose gel without ethidium bromide. The left panel illustrates the fluorescein signal from the labeled RNA in the unstained gel. The right panel represents the same gel after ethidium bromide staining of the RNA.
Figure 3
Figure 3. Generation of Sensitive FISH Probes. Multicolor chromosome PAINT analysis using Label IT® Biotin labeled human chromosome 7 (Streptavidin-Cy™ 3 conjugate detection) and Label IT® Fluorescein labeled human chromosome 6 on a Jurkat metaphase spread, with DAPI stained chromosomes in blue.
Figure 4
Figure 4. Labeling Density can be Easily Controlled with the Label IT® Reagents. Plasmid DNA was labeled with increasing ratios (w:w) of Label IT® CX-Rhodamine to DNA. The extent of labeling was estimated, and normalized to the amount of recovered DNA (# labels/100 base pairs), using two different criteria: fluorescence intensity of the attached rhodamine (excitation at 585 nm, emission at 610 nm), and visible absorbance at 585 nm. The significance of quenching, at the higher labeling ratios, becomes apparent in the fluorimetric estimations of labeling density.
Fluorophore Excitation Wavelength (nm) Emission Wavelength (nm)
Cy™3 550 570
Cy™5 649 670
CX-Rhodamine 576 597
TM-Rhodamine 546 576
Fluorescein 492 518
Figure 5. Excitation and Emission Wavelengths of Fluorescent Label IT® Reagents.

Note: All the Label IT® Reagents use the same technology, but each attach different fluorophores with distinct excitation and emission spectra.