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NEW! Label IT® miRNA Labeling Kits, Version 2

Rapid and sensitive non-enzymatic labeling of microRNA (miRNA) in total or enriched RNA samples for microarray analysis

Product No.QuantityPriceAdd to
Label IT® miRNA Labeling Kit, Version 2, Biotin
MIR 941010 reactions $282.00
MIR 945050 reactions $1,070.00
Label IT® miRNA Labeling Kit, Version 2, Cy™3
MIR 951010 reactions $282.00
MIR 955050 reactions $1,070.00
Label IT® miRNA Labeling Kit, Version 2, Cy™3/Cy™5
MIR 93052 x 5 reactions $282.00
MIR 93252 x 25 reactions $1,070.00
Label IT® miRNA Labeling Kit, Version 2, Cy™5
MIR 961010 reactions $282.00
MIR 965050 reactions $1,070.00
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.

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Protocol
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  • Sensitive - Detects subattomolar amounts of miRNA species.
  • Accurate - Labels all miRNAs present in the sample.
  • Universal - Labels total or enriched RNA from fresh, frozen or FFPE tissues including plants.
  • Sequence Independent Labeling - Labels all nucleotides with equal efficiency.
  • Save Time - Simple, one hour protocol.

Kit Components:
Each kit includes the Label IT® miRNA Cy™3 and Cy™5 Reagents, Reconstitution Solution, 10X Labeling Buffer M, 10X Stop Solution, Purification Columns (with Collection Tubes), Column Binding Buffer, Column Wash Buffer, Column Elution Solution, and Elution Tubes. This kit also contains a 2X Hybridization Solution.

Data

Figure 1: The Label IT miRNA Labeling Kits.
Figure 2: The Label IT miRNA Labeling Kit is More Sensitive Than NCode™.
Figure 3: Accurately Detect All miRNAs in Your Sample.

Figure 1
Figure 1. The Label IT® miRNA Labeling Kits. The Label IT Cy™3, Cy™5, and biotin reagents are composed of three regions: the Cy3, Cy5 or biotin label (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT reagents to any reactive heteroatom within miRNAs. The covalent attachment of the Label IT reagents to miRNA does not alter the structure or downstream hybridization performance of the miRNA, and thus, once labeled, the miRNA samples can be hybridized to miRNA-specific microarrays and analyzed to determine the miRNA expression profile.
Figure 2
Figure 2. The Label IT® miRNA Labeling Kit is More Sensitive Than NCode™. A pool of 699 synthetic miRNAs was labeled and hybridized to the NCode™ Multi-Species miRNA Microarray V2 at varying input amounts. Features with signal greater than 3X background were considered detected.
Figure 3
Figure 3. Accurately Detect All miRNAs in Your Sample. Representative array images of specific miRNAs missed by enzymatic labeling. Yellow tests were performed using synthetic pools of miRNAs (2.5 fmol of each miRNA) labeled with either chemical (Label IT®) or enzymatic (NCode Rapid) labeling, and hybridized to the NCode™ Multi-Species miRNA Microarray.