Font Size: Font Size: 11px Font Size: 12px Font Size: 14px
 
Current Items: 0
Subtotal: $0
 
Download Catalog (pdf)
Request Literature
Corporate News
Quick Search QUICK SEARCH

Ingenio™ Electroporation Products

High efficiency electroporation using any instrument

Resources

Protocol
MSDS
Ingenio Pulse Condition Recommendations
Request a Sample
Contact Technical Support

View Data
Product No.QuantityPriceAdd to
Ingenio™ Electroporation Kit, 0.2 cm cuvettes, Ideal with amaxa Nucleofector Devices
MIR 501126.25 ml solution with 25 cuvettes and cell droppers$200.00
MIR 5011512.5 ml solution with 50 cuvettes and cell droppers$350.00
MIR 5011825 ml solution with 100 cuvettes and cell droppers$650.00
Ingenio™ Electroporation Kit, 0.4 cm cuvettes, Ideal with all other Electroporation Instruments such as the Bio-Rad GenePulser or BTX instruments
MIR 501136.25 ml solution with 25 cuvettes and cell droppers$200.00
MIR 5011612.5 ml solution with 50 cuvettes and cell droppers$350.00
MIR 5011925 ml solution with 100 cuvettes and cell droppers$650.00
Ingenio™ Electroporation Solution1
MIR 501116.25 ml$125.00
MIR 5011412.5 ml$200.00
MIR 5011725 ml$350.00
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.
1 Ingenio Solution is compatible with all electroporators including amaxa's Nucleofector® Device.

Recommended Conditions for Ingenio™ Electroporation
RNAi Research Using Ingenio™ Kits
amaxa Comparison
Comparison to Other Solutions

  • High efficiency electroporation of hard to transfect cell lines and primary cells
  • Compatible with all electroporation devices including Lonza-amaxa®, Bio-Rad® or Harvard BTX® 
  • Ideal for plasmid or siRNA electroporation
  • Save money and reduce research costs while maximizing results
Mirus Bio has developed the Ingenio™ Electroporation Solution to facilitate efficient and reliable delivery of nucleic acids to eukaryotic cells traditionally resistant to chemical transfection. Ingenio is a broad spectrum solution that supports high efficiency electroporation with minimal toxicity. It replaces standard electroporation solutions including phosphate buffered saline and serum-free media. Ingenio is compatible with multiple instruments and facilitates a wide range of applications requiring nucleic acid delivery to cells. The Ingenio solution is available alone and as part of a complete kit with cuvettes and cell droppers.


Recent Citations

OCRL1 function in renal epithelial membrane traffic
Shanshan Cui, Christopher J. Guerriero, Christina M. Szalinski, Carol L. Kinlough, Rebecca P. Hughey, and Ora A Weisz
Am J Physiol Renal Physiol. 2010 Feb;298(2):F335-45. Epub 2009 Nov 25.

Porcine reproductive and respiratory syndrome virus non structural protein 1 modulates host innate immune response by antagonizing IRF3 activation
Lalit K. Beura, Saumendra N. Sarkar, Byungjoon Kwon, Sakthivel Subramaniam, Clinton Jones, Asit K. Pattnaik, and Fernando A. Osorio
J Virol. 2010 Feb;84(3):1574-84. Epub 2009 Nov 18.

Data

Figure 1: Ingenio Solution Provides Comparable Efficiency on amaxa's Nucleofector Device
Figure 2: Ingenio Solution used with Gene Pulser Electroporator Yields Comparable High Efficiency to the amaxa Nucleofector II System
Figure 3: Ingenio Provides High Efficiency Electroporation of Hard to Transfect Cell Lines
Figure 4: Potent siRNA Knockdown of Transiently Expressed Firefly Luciferase Using the Ingenio Electroporation Solution
Figure 5: Efficient miRNA Mediated Knockdown Using Ingenio
Figure 6: Ingenio Outperforms Other Electroporation Solutions
Figure 7: High Cell Viability with Ingenio

Figure 1
Figure 1. Ingenio™ Solution Provides Comparable Efficiency on amaxa's Nucleofector® Device. Cells were electroporated in parallel with an EGFP reporter vector. Two electroporators were used with different electroporation kits: the Ingenio™ Electroporation Kit was used in the Gene Pulser Xcell™ Eukaryotic System (Bio-Rad) and the amaxa Nucleofector® II Device (Lonza); the amaxa Nucleofector® Kit V was used in the amaxa Nucleofector® II Device, all according to manufacturer's recommendations. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population.
Figure 2
Figure 2. Ingenio Solution used with Gene Pulser Electroporator Yields Comparable High Efficiency to the amaxa Nucleofector II System. HL-60, Jurkat E6-1, K562, THP-1 and SK-N-MC cells were electroporated with the same EGFP reporter on the same day, using either Ingenio™ Electroporation Solution in the Gene Pulser XCell (Bio-Rad) (red bars) or the amaxa Solution V in amaxa Nucleofector® II (Lonza) (light grey bars).
Figure 3
Figure 3. Ingenio Provides High Efficiency Electroporation of Hard to Transfect Cell Lines. Cells were electroporated with an EGFP reporter vector using the Ingenio™ Electroporation Solution and a Gene Pulser Xcell™ Eukaryotic System. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. Experiments were performed in triplicate on three separate days and the data averaged.
Figure 4
Figure 4. Potent siRNA Knockdown of Transiently Expressed Firefly Luciferase Using the Ingenio™ Electroporation Solution. siRNA and plasmid DNA were co-electroporated with the indicated cell lines with the Ingenio™ electroporation solution in 0.2 cm cuvettes using either the Gene Pulser® (Bio-Rad) or the amaxa Nucleofector® II (Lonza). 10µg/ml of plasmid encoding Firefly luciferase was co-electroporated with 250nM of either non-targeting siRNA control (Dharmacon Non-Targeting siRNA #1) or GL3 siRNA into Jurkat E6-1, HeLa and CHO-K1 cells. Twenty-four hours post electroporation, cells were harvested and assayed for luciferase activity. Data from independent experiments performed on different days were averaged then scaled to non-targeting siRNA control and are represented as a percentage of the control.

Pulse conditions used:

(1) amaxa Nucleofector® program:
     a. Jurkat E6-1: X-001 with 10 x 106 cells/ml
     b. HeLa: I-013 with 3 x 106 cells/ml
     c. CHO-K1: U-023 with 5 x 106 cells/ml

(2) Bio-Rad Gene Pulser® Exponential Decay setting:
     a. Jurkat: 150V, 950µF with 10 x 106 cells/ml
     b. HeLa : 130V, 950µF with 3 x 106 cells/ml
     c. CHO-K1: 150V, 900µF with 5 x 106 cells/ml
Figure 5
Figure 5. Efficient miRNA Mediated Knockdown Using Ingenio. psiCHECK2 plasmid (Promega) cloned with target sequence for miR-143 was co-electroporated with miR-143 microRNA (Ambion) or non-targeting control microRNA in Ingenio™ Electroporation Solution using the Gene Pulser Xcell (Bio-Rad). Cells were harvested at 24 hours post-electroporation and assayed using the Dual-Luciferase System (Promega). Activity is represented as a percentage of knockdown relative to the control.
Figure 6
Figure 6. Ingenio Outperforms Other Electroporation Solutions. Cells were electroporated in parallel with an EGFP reporter vector using either Ingenio™ Electroporation Solution or PBS on the Gene Pulser Xcell™ Eukaryotic System. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. Experiments were performed in triplicate on three separate days and the data averaged.
Figure 7
Figure 7. High Cell Viability with Ingenio. DNA was electroporated into cells using either Ingenio™ Electroporation Solution or PBS on the Gene Pulser Xcell™ Eukaryotic System. Twenty-four hours post-electroporation, cells were assayed for viablility by propidium iodide staining and flow cytometry analysis. Experiments were performed in triplicate on three separate days and the data averaged.