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Ingenio® Electroporation Products

High efficiency electroporation using any instrument

Product No.QuantityPriceAdd to Cart
Ingenio® Electroporation Kit for Lonza-amaxa Nucleofector devices (0.2 cm cuvettes and cell droppers)
MIR 5011225 reactions$208.00
MIR 5011550 reactions$364.00
MIR 50118100 reactions$676.00
Ingenio® Electroporation Kits for all other electroporators, such as Bio-Rad and Harvard-BTX (0.4 cm cuvettes and cell droppers)
MIR 5011325 reactions$208.00
MIR 5011650 reactions$364.00
MIR 50119100 reactions$676.00
Ingenio® Electroporation Solution
MIR 5011125 reactions$130.00
MIR 5011450 reactions$208.00
MIR 50117100 reactions$364.00
To inquire about bulk pricing, please call 888-530-0801.
International inquiries please call +1-608-441-2852.
Note: Ingenio Solution is compatible with all electroporators including Amaxa's Nucleofector® Device.

Resources

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Frequently Asked Questions
Ingenio Pulse Condition Recommendations
Poster: Electroporating Hard to Transfect Cells...
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Ingenio Pulse Condition Recommendations (pdf)
RNAi Research Using Ingenio Kits
Amaxa Comparison
Comparison to Other Solutions


  • High efficiency electroporation of hard to transfect cell lines and primary cells
  • Compatible with all electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®
  • Ideal for plasmid or siRNA electroporation
  • Save money and reduce research costs while maximizing results
Mirus Bio has developed the Ingenio® Electroporation Solution to facilitate efficient and reliable delivery of nucleic acids to eukaryotic cells traditionally resistant to chemical transfection. Ingenio is a broad spectrum solution that supports high efficiency electroporation with minimal toxicity. It replaces standard electroporation solutions including phosphate buffered saline and serum-free media. Ingenio is compatible with multiple instruments and facilitates a wide range of applications requiring nucleic acid delivery to cells. The Ingenio solution is available alone and as part of a complete kit with cuvettes and cell droppers.

Data

Figure 1: Ingenio Solution Provides Comparable Efficiency on amaxa Nucleofector Device
Figure 2: Efficient Plasmid DNA Delivery Using Ingenio Solution with Amaxa Nucleofector Device
Figure 3: Ingenio Outperforms Other Electroporation Solutions
Figure 4: High Cell Viability with Ingenio
Figure 5: Ingenio Provides High Efficiency Electroporation of Hard to Transfect Cell Lines
Figure 6: Potent siRNA Knockdown of Transiently Expressed Firefly Luciferase Using the Ingenio Electroporation Solution

Figure 1:Ingenio Solution Provides Comparable Efficiency on amaxa Nucleofector Device
Figure 1. Ingenio® Solution Provides Comparable Efficiency on amaxa Nucleofector® Device. Cells were electroporated in parallel with an EGFP reporter vector and assayed at 24 hours post-electroporation by flow cytometry. Two electroporators were used with different electroporation kits: the Ingenio Electroporation Kit was used in the Gene Pulser Xcell™ Eukaryotic System (Bio-Rad) and the Amaxa Nucleofector II Device (Lonza); the Amaxa Nucleofector Kit V was used in the Amaxa Nucleofector II Device, all according to manufacturer's recommendations.
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Figure 2:Efficient Plasmid DNA Delivery Using Ingenio Solution with Amaxa Nucleofector Device
*Primary cell types

Figure 2. Efficient Plasmid DNA Delivery Using Ingenio® Solution with Amaxa Nucleofector Device. Ingenio Electroporation Kits were used to transfect indicated cell types using Amaxa® Nucleofector® II Device. Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population. See the recommended experimental conditions.
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Figure 3:Ingenio Outperforms Other Electroporation Solutions
Figure 3. Ingenio® Outperforms Other Electroporation Solutions. Cells were electroporated in parallel with an EGFP reporter vector using either Ingenio Electroporation Solution, PBS or the Gene Pulser® Electroporation Buffer (Bio-Rad) on the Gene Pulser Xcell™ Eukaryotic System. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. Experiments were performed in triplicate on three separate days and the data averaged.
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Figure 4:High Cell Viability with Ingenio
Figure 4. High Cell Viability with Ingenio®. DNA was electroporated into cells using either Ingenio Electroporation Solution, PBS or Gene Pulser Electroporation Buffer (Bio-Rad) and the Gene Pulser Xcell™ Eukaryotic System. Twenty-four hours post-electroporation, cells were assayed for viablility by propidium iodide staining and flow cytometry analysis. Experiments were performed in triplicate on three separate days and the data averaged.
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Figure 5:Ingenio Provides High Efficiency Electroporation of Hard to Transfect Cell Lines
Figure 5. Using Standard Electroporators, Ingenio® Provides High Efficiency Electroporation of Hard to Transfect Cell Lines. Cells were electroporated with an EGFP reporter vector using the Ingenio Electroporation Solution and a Gene Pulser Xcell™ Eukaryotic System. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. Experiments were performed in triplicate on three separate days and the data averaged. See the recommended pulse conditions.
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Figure 6:Potent siRNA Knockdown of Transiently Expressed Firefly Luciferase Using the Ingenio Electroporation Solution
Figure 6. Potent siRNA Knockdown of Transiently Expressed Firefly Luciferase Using the Ingenio® Electroporation Solution. siRNA and plasmid DNA were co-electroporated with the indicated cell lines with the Ingenio electroporation solution in 0.2 cm cuvettes using either the Gene Pulser® (Bio-Rad) or the Amaxa Nucleofector® II (Lonza). 10µg/ml of plasmid encoding Firefly luciferase was co-electroporated with 250nM of either non-targeting siRNA control (Dharmacon Non-Targeting siRNA #1) or GL3 siRNA into Jurkat E6-1, HeLa and CHO-K1 cells. Twenty-four hours post electroporation, cells were harvested and assayed for luciferase activity. Data from independent experiments performed on different days were averaged then scaled to non-targeting siRNA control and are represented as a percentage of the control.

Pulse conditions used:

(1) Amaxa Nucleofector® program:
a. Jurkat E6-1: X-001 with 10 x 106 cells/ml
b. HeLa: I-013 with 3 x 106 cells/ml
c. CHO-K1: U-023 with 5 x 106 cells/ml

(2) Bio-Rad Gene Pulser® Exponential Decay setting:
a. Jurkat: 150V, 950µF with 10 x 106 cells/ml
b. HeLa : 130V, 950µF with 3 x 106 cells/ml
c. CHO-K1: 150V, 900µF with 5 x 106 cells/ml
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