Ingenio® Electroporation Products

Figure 1. High Efficiency Plasmid DNA Electroporation of Human Induced Pluripotent Stem (iPS) Cells using Ingenio®. The Ingenio® Electroporation Kit was used to transfect 2 x 10⁶ iPS cells on the Amaxa® Nucleofector® II Device. Cells were electroporated with 8 µg ZsGreen expressing plasmid (Clontech) in 100 µl and plated in 6-well plates at 0.33 x 10⁶ cells/well. Cells were visualized 24 hours post-transfection and imaged under 4X objective with an Olympus IX71® Inverted Microscope. Images are (A) phase contrast, and (B) green fluorescence. Cells were also assayed 24 hours post-transfection on an Accuri® Cytometer. The histogram (C) shows unelectroporated cells (black line) compared to cells electroporated with plasmid using the Ingenio Electroporation Kit (green line). See more information on stem cell applications.

Data courtesy of

Figure 2. Ingenio^® Solution Provides Comparable Efficiency on Amaxa Nucleofector^® Device. Cells were electroporated in parallel with an EGFP reporter vector and assayed at 24 hours post-electroporation by flow cytometry. Two electroporators were used with different electroporation kits: the Ingenio Electroporation Kit was used in the Gene Pulser Xcell™ Eukaryotic System (Bio-Rad) and the Amaxa Nucleofector II Device (Lonza); the Amaxa Nucleofector Kit V was used in the Amaxa Nucleofector II Device, all according to manufacturer's recommendations.

*Primary cell types

Figure 3. Efficient Plasmid DNA Delivery Using Ingenio^® Solution with Amaxa Nucleofector Device. Ingenio Electroporation Kits were used to transfect indicated cell types using Amaxa® Nucleofector® II Device. Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population. See the recommended experimental conditions.

Figure 4. Ingenio^® Outperforms Other Electroporation Solutions in Efficiency and Viability. Cells were electroporated in parallel with an EGFP reporter vector using either Ingenio Electroporation Solution, PBS or the Gene Pulser® Electroporation Buffer (Bio-Rad) on the Gene Pulser Xcell™ Eukaryotic System. (A) EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. (B) Cells were assayed for viablility by propidium iodide staining and flow cytometry analysis. Experiments were performed in triplicate on three separate days and the data averaged.

Figure 5. Using Standard Electroporators, Ingenio^® Provides High Efficiency Electroporation of Hard to Transfect Cell Lines. Cells were electroporated with an EGFP reporter vector using the Ingenio Electroporation Solution and a Gene Pulser Xcell™ Eukaryotic System. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. Experiments were performed in triplicate on three separate days and the data averaged. See the recommended pulse conditions.