Label IT® Plasmid Delivery Controls

Fluorescently labeled plasmid ideal for In Vitro and In Vivo tracking

Resources

Protocol
MSDS
Label IT Excitation and Emission Spectra
Contact Technical Support

• Sensitive - Easily visualize transfected cells and assess delivery efficiency using fluorescent microscopy.
• Compatible - Suitable for co-delivery experiments with functional plasmids.
• Ease of Use - Supplied as a ready to use 0.5 mg/ml concentration for both In Vitro and In Vivo tracking studies.

Data

Figure 1. The Label IT® Cy3™ Plasmid Delivery Control Allows Quick Assessment of Delivery Efficiency. HeLa cells were transfected in serum-containing media with the Label IT® Cy™3 Plasmid Delivery Control (red) using the TransIT®-LT1 Transfection Reagent. Twenty-four hours post-transfection, the cells were fixed, then counterstained to locate the nuclei (blue) and to stain the actin (green).

Back to Top

 

Figure 2. Visualization of Label IT® Fluorescein Plasmid Delivery Control in Liver Sections Following Tail Vein Injection. TransIT®-QR Hydrodynamic Delivery Solution was used to deliver 25 µg of Label IT® Fluorescein Plasmid Control (green) to a mouse using hydrodynamic delivery via the tail vein. One hour post-injection the liver was harvested. Sections were fixed to stain the nuclei (blue) and the actin (red).

Back to Top