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Electroporation
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Compare TransIT®-mRNA Transfection Kit to Electroporation:
- Superior Performance
- Easier to Use
- Saves Valuable Time
- Saves Valuable Resources
10X Better Performance using 2.5X Less RNA
Huh7 cells were transfected with 2 µg of a yellow fever virus-luciferase expressing RNA replicon (YFV-luc) using the TransIT®-mRNA Transfection Kit or electroporated in parallel with 5 µg of the YFV-luc RNA replicon using an ECM630 Electroporator (BTX) under optimal conditions. Twenty-four hours later, the cells were harvested and assayed for luciferase activity and total protein content. Luciferase activity was normalized to total protein content to correct for differences in cell number between samples(1).
(1) Gonzalez, G. et al. (2007) Selection of an Optimal RNA Transfection Reagent and Comparison to Electroporation for the Delivery of viral RNA. J. Virological. Methods. 145:14-21
Simple Protocol Saves Over 4 Hours
Save Time and Valuable Resources
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TransIT®-mRNA Kit
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Electroporation
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| Cell Number |
~3.8 x 105
(per well of 6-well plate)
Scalable based on culture vessel surface area |
~5 x 106
(Plated in 6-well plate after electroporation) |
| RNA Amount |
2.5 µg
(per well of 6-well plate)
Scalable based on culture vessel surface area |
~5 µg
(per standard electroporation) |
| Serum Compatibility |
Compatible
- Enhances cell viability
- Simplifies protocol |
Incompatible
- Requires serum free conditions
- Requires media changes |
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Prove It To Yourself
- Request Your FREE TransIT-mRNA Sample Today - MORE
- Check out these publications citing TransIT®-mRNA use:
Gonzalez et. al, (2007) Selection of an Optimal RNA Transfection Reagent and Comparison to Electroporation for the Delivery of Viral RNA. J. Virological Methods. 145:14-21 (Direct comparison to DMRIE-C, TransMessenger™, and electroporation)
Meis and Meis, (2007) The New mScript™ mRNA Production System - Efficient mRNA Transcription, Capping and Tailing for High Yields of Active Protein. EPICENTRE Forum. 14(1):4-5 (Efficient mRNA delivery into HeLa, NIH3T3 and BDCM cell lines) http://www.epibio.com/pdfforum/14_1.pdf
Duan and Jefcoate. (2007) The Predominant cAMP-stimulated 3.5 kb StAR mRNA Contains Specific Sequence Elements in the Extended 3'UTR that Confer High Basal Instability. J. of Mol. Endocrin. 38:159-179 (mRNA transfection followed by mRNA stability measurements)
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