The TransIT®-mRNA Transfection Kit
The Best Choice for High Efficiency, Low Toxicity RNA Transfections
- High Efficiency RNA Delivery – Ensures experimental success by effectively transfecting RNA into a large percentage of the cell population
- Serum Compatible
- Simplifies the transfection protocol
- Saves valuable time
- Increases cell viability thereby minimizing experimental biases due to alterations in cell physiology
- Transfects Various RNA Types and Sizes – Ideal for multiple applications such as viral production and replication studies and short-term protein expression from mRNA
High Efficiency RNA Transfection of Mammalian Cell Lines
Cells were transfected with a capped, polyadenylated EGFP mRNA using the TransIT®-mRNA Transfection Kit and analyzed by flow cytometry 18 hours post-transfection to identify the EGFP expressing cells.
TransIT®-mRNA Kit Successfully Delivers Many Viral RNAs |
| Virus |
Application |
RNA Size |
Laboratory, Institution |
| Feline Calicivirus |
Virus Production |
8 kb |
Hardy, Montana State University |
| Hepatitis C Virus |
Replicon |
9 kb |
Striker, University of Wisconsin |
| Murine Coronavirus |
Viral Production |
32 kb |
Baker, Loyola University Chicago |
| Poliovirus |
Virus Production |
7.5 kb |
Ehrenfeld, LID, NIAID, NIH |
| Yellow Fever Virus |
Replicon |
6 kb |
Striker, University of Wisconsin |
| Yellow Fever Virus |
Virus Production |
9 kb |
Striker, University of Wisconsin |
MHV Coronavirus Induced Syncytia Formed After
Transfection of a 32 kb MHV Genomic RNA |
No RNA Control
 |
MHV RNA
 |
| Data courtesy of Mark Clementz, Baker Lab, Loyola University Chicago |
Easy Transfection Protocol in Serum Containing Growth Media
Prove It To Yourself
- Request Your FREE TransIT-mRNA Sample Today - MORE
- View electroporation comparison - MORE
- View TransMessenger™ comparison - MORE
- Check out these publications citing TransIT®-mRNA use:
Gonzalez et. al, (2007) Selection of an Optimal RNA Transfection Reagent and Comparison to Electroporation for the Delivery of Viral RNA. J. Virological Methods. 145:14-21 (Direct comparison to DMRIE-C, TransMessenger™, and electroporation)
Meis and Meis, (2007) The New mScript™ mRNA Production System - Efficient mRNA Transcription, Capping and Tailing for High Yields of Active Protein. EPICENTRE Forum. 14(1):4-5 (Efficient mRNA delivery into HeLa, NIH3T3 and BDCM cell lines) http://www.epibio.com/pdfforum/14_1.pdf
Duan and Jefcoate. (2007) The Predominant cAMP-stimulated 3.5 kb StAR mRNA Contains Specific Sequence Elements in the Extended 3'UTR that Confer High Basal Instability. J. of Mol. Endocrin. 38:159-179 (mRNA transfection followed by mRNA stability measurements)
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