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The TransIT®-mRNA Transfection Kit

The Best Choice for High Efficiency, Low Toxicity RNA Transfections

  • High Efficiency RNA Delivery - Ensures experimental success by effectively transfecting RNA into a large percentage of the cell population
  • Serum Compatible
    - Simplifies the transfection protocol
    - Saves valuable time
    - Increases cell viability thereby minimizing experimental biases due to alterations in cell physiology
  • Transfects Various RNA Types and Sizes - Ideal for multiple applications such as viral production and replication studies and short-term protein expression from mRNA
High Efficiency RNA Transfection of Mammalian Cell Lines
Cells were transfected with a capped, polyadenylated EGFP mRNA using the TransIT®-mRNA Transfection Kit and analyzed by flow cytometry 18 hours post-transfection to identify the EGFP expressing cells.
 
Virus Application RNA Size Laboratory, Institution
Feline Calicivirus Virus Production 8 kb Hardy, Montana State University
Hepatitis C Virus Replicon 9 kb Striker, University of Wisconsin
Murine Coronavirus Virus Production 32 kb Baker, Loyola University Chicago
Poliovirus Virus Production 7.5 kb Ehrenfeld, LID, NIAID, NIH
Yellow Fever Virus Replicon 6 kb Striker, University of Wisconsin
Yellow Fever Virus Virus Production 9 kb Striker, University of Wisconsin
 
No RNA Control (A)
 MHV RNA (B)
No RNA Control MHV CPE

Data courtesy of Mark Clementz, Baker Lab, Loyola University Chicago
 
Easy Transfection Protocol in Serum Containing Growth Media
 
Prove It To Yourself
  • Request Your FREE TransIT-mRNA Sample Today - MORE
  • View electroporation comparison - MORE
  • View TransMessenger™ comparison - MORE
  • Check out these publications citing TransIT®-mRNA use:

    Gonzalez et. al, (2007) Selection of an Optimal RNA Transfection Reagent and Comparison to Electroporation for the Delivery of Viral RNA. J. Virological Methods. 145:14-21 (Direct comparison to DMRIE-C, TransMessenger™, and electroporation)

    Meis and Meis, (2007) The New mScript™ mRNA Production System - Efficient mRNA Transcription, Capping and Tailing for High Yields of Active Protein. EPICENTRE Forum. 14(1):4-5 (Efficient mRNA delivery into HeLa, NIH3T3 and BDCM cell lines)

    Duan and Jefcoate. (2007) The Predominant cAMP-stimulated 3.5 kb StAR mRNA Contains Specific Sequence Elements in the Extended 3'UTR that Confer High Basal Instability. J. of Mol. Endocrin. 38:159-179 (mRNA transfection followed by mRNA stability measurements)