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The Label IT® miRNA Labeling Kits – The Best Choice for Accurate, Sensitive and Reproducible MicroRNA (miRNA) Microarray Expression Profiling

  • Accurate and Reproducible – Confidently detect all miRNAs present in a sample including those our competitors systematically miss
  • Sensitive Detection of Subfemtomole miRNA Amounts – Detect low abundance miRNAs and generate complete expression profiles
  • Simple, Rapid Labeling – One step, direct chemical labeling reaction completed in only two hours saving valuable time and reducing chance of experimental error

Accurate - miRNA Expression Profiles Corroborate Northern Blotting Results

Figure 1

miRNA-enriched samples from mouse brain and heart were labeled with Cy™3 (brain) or Cy™5 (heart) using the Label IT® miRNA Labeling Kit. The labeled samples were pooled and hybridized to duplicate miRMAX™ miRNA-specific microarrays (Rutgers). (A) Representative field from a hybridized array. (B) Average corrected Cy™3 (green bars, brain expression) and Cy™5 (red bars, heart expression) signals for 12 candidate miRNA features. (C) Predominant miRNA expression pattern determined by northern blotting (H = heart, B = brain) and published previously (Sempere et. al. 2004. Genome Biology 5(3): R13).

Easy to Use – 1 Step Direct Chemical Labeling in Only 2 Hours

Figure 2

The Label IT® miRNA Labeling Reagents are composed of a fluorophore (or biotin) attached to the positively charged Label IT® linker and reactive alkylating group. The Label IT® Reagents covalently attach fluorophores (or biotin) to any reactive heteroatom in miRNAs without affecting downstream hybridization performance. miRNA labeling and purification can be completed in only two hours.

- Purchase any Label IT® miRNA Labeling Kit and receive 25% off > more
- View comparison to Ambion’s mirVana™ and Invitrogen’s NCode™ Labeling Kits > more
- View protocol comparisons > more

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