The Ingenio^® Kits Effectively Electroporate siRNA on the Amaxa Nucleofector^® and the Gene Pulser^® Electroporators

siRNA and plasmid DNA were co-electroporated with the indicated cell lines with the Ingenio^® electroporation solution in 0.2 cm cuvettes using either the Gene Pulser® (Bio-Rad) or the Amaxa Nucleofector® II (Lonza). 10µg/ml of plasmid encoding Firefly luciferase was co-electroporated with 250nM of either non-targeting siRNA control (Dharmacon Non-Targeting siRNA #1) or GL3 siRNA into Jurkat E6-1, HeLa and CHO-K1 cells. Twenty-four hours post electroporation, cells were harvested and assayed for luciferase activity. Data from independent experiments performed on different days were averaged then scaled to non-targeting siRNA control and are represented as a percentage of the control.

Pulse conditions used:

(1) Amaxa Nucleofector® program:
a. Jurkat E6-1: X-001 with 10 x 10⁶ cells/ml
b. HeLa: I-013 with 3 x 10⁶ cells/ml
c. CHO-K1: U-023 with 5 x 10⁶ cells/ml

(2) Bio-Rad Gene Pulser® Exponential Decay setting:
a. Jurkat: 150V, 950µF with 10 x 10⁶ cells/ml
b. HeLa : 130V, 950µF with 3 x 10⁶ cells/ml
c. CHO-K1: 150V, 900µF with 5 x 10⁶ cells/ml