High Electroporation Efficiency using Ingenio^® Solution

Cells were electroporated in parallel with an EGFP reporter vector using either Ingenio Electroporation Solution, PBS or the Gene Pulser® Electroporation Buffer (Bio-Rad) on the Gene Pulser Xcell™ Eukaryotic System. (A) EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. (B) Cells were assayed for viablility by propidium iodide staining and flow cytometry analysis. Experiments were performed in triplicate on three separate days and the data averaged.