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Same Results Less Costs using Ingenio® with Amaxa Nucleofector® II

Ingenio® Solution Provides Comparable Efficiency on Amaxa Nucleofector®

Similar Performance on Amaxa Nucleofector® Device
Cells were electroporated in parallel with an EGFP reporter vector using a Nucleofector® II Device (Amaxa) and either the Ingenio® Electroporation Solution or the Nucleofector Kit V (Amaxa) according to Amaxa’s recommended protocol for each cell line [HL-60 (T-019), Jurkat E6-1(X-001), K-562 (T-016), THP-1 (V-001), SK-N-MC (A-023), and MCF-7 (P020)]. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population.
 

Ingenio® Kits provide a Cost-Effective and Stable Alternative to Nucleofector® Kitsa

Product Shelf Life Cost/Electroporationb Savings
Amaxa Nucleofector® Kit V (VCA-1003) 3 months
$14.28 -
Ingenio® Electroporation Kit (MIR 50112) 1 year
$8.32 42%

a
Compatible with Amaxa Nucleofector II device

b
Based on 2012 U.S. List prices from company websites and protocol recommendations (25 RXN kit).
 

Ingenio® Solution Shows Similar Results with Amaxa® Nucleofector and Other Electroporators

Ingenio® and Gene Pulser® Electroporator Yield Comparable Performance to amaxa Nucleofector® System
Ingenio® Solution used with Gene Pulser Electroporator Yields Comparable High Efficiency to the Amaxa Nucleofector II System: HL-60, Jurkat E6-1, K562, THP-1 and SK-N-MC cells were electroporated with the same EGFP reporter on the same day, using either Ingenio Electroporation Solution in the Gene Pulser XCell (Bio-Rad) (red bars) or the Amaxa Solution V in Amaxa Nucleofector® II (Lonza) (light grey bars).
 

Ingenio® Resources

Reagent Agent Don't See Your Cell Type? Consult Reagent Agent® Transfection Database
Citation Database: Check if Ingenio® has been used by other researchers to transfect your cell type
Technical Support: Speak directly with a transfection expert
Ingenio Product Webpage: View more product details


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