Same Results Less Costs using Ingenio^® with Amaxa Nucleofector^® II

Cells were electroporated in parallel with an EGFP reporter vector using a Nucleofector® II Device (Amaxa) and either the Ingenio® Electroporation Solution or the Nucleofector Kit V (Amaxa) according to Amaxa’s recommended protocol for each cell line [HL-60 (T-019), Jurkat E6-1(X-001), K-562 (T-016), THP-1 (V-001), SK-N-MC (A-023), and MCF-7 (P020)]. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population.

Ingenio^® Solution used with Gene Pulser Electroporator Yields Comparable High Efficiency to the Amaxa Nucleofector II System: HL-60, Jurkat E6-1, K562, THP-1 and SK-N-MC cells were electroporated with the same EGFP reporter on the same day, using either Ingenio Electroporation Solution in the Gene Pulser XCell (Bio-Rad) (red bars) or the Amaxa Solution V in Amaxa Nucleofector® II (Lonza) (light grey bars).